A Method for Assisting Preparation of Silver Nanoparticles (Ag NPs) by Ginger Extract

专利摘要:
The invention discloses a method for assisting preparation of silver nanoparticles (Ag NPs) by ginger extract which includes the following steps: pulverize the cut ginger slices, weigh a certain quality of ginger powder, select distilled water as a solvent to heat and reflux for a period of time. After 5 centrifugation, it is filtered to obtain the ginger extract. Take a certain volume of ginger extract and mix with a certain volume and concentration of silver nitrate solution, stir for a period of time at room temperature, and use separate ginger extract and silver nitrate solution as controls to test the UV absorption intensity and antibacterial effect. The method adopted by the present invention is relatively simple, convenient in experimental operation steps, low in cost, environmentally friendly, simpler than 10 traditional synthesis methods, and the synthesized Ag NPs have good antibacterial effects. 6
公开号:NL2028297A
申请号:NL2028297
申请日:2021-05-26
公开日:2021-08-30
发明作者:Fang Jing；Song Jiming；Dong Zhuangzhuang
申请人:Univ Anhui；
IPC主号:

专利说明:

[0001] [0001] The present invention relates to the field of preparation of silver nanomaterials and antibacterial applications, in particular to a method for assisting preparation of Ag NPs by ginger extract and its application in antibacterial aspects. Background Technology
[0002] [0002] Ag NPs is a simple substance of metallic silver with a particle size of nanometers. Ag NPs have surface effects, quantum size effects and other effects, as well as good electrical conductivity, which makes them occupy an extremely important position in microelectronics applications, medical applications, surface enhanced Raman applications, catalytic materials, green home appliances and furniture products, etc. Ag NPs is most commonly used as an antibacterial material. Small-sized Ag NPs will directly enter the bacteria and combine with oxygen metabolism enzymes, which can kill most of the bacteria, fungi, molds, spores and other microorganisms that come into contact with it.
[0003] [0003] Common preparation methods of Ag NPs include chemical methods, physical methods, and biological reduction methods. Chemical methods usually use reducing substances such as citric acid, sodium borohydride, and ascorbic acid as reducing agents, and assist a certain surfactant to adjust the morphology of the particles. It is a commonly used method for synthesizing Ag NPs. The physical methods mainly include mechanical ball milling, laser burning, and ultrasonic. The preparation process is simple, and the purity of the prepared Ag NPs is relatively high, but the preparation conditions are not easy to control. It has strict requirements on equipment and high production costs. In recent years, the use of natural plant extracts to prepare Ag NPs has received more attention. The natural plant extracts act as both a reducing agent and a regulator of the morphology of silver particles in the reaction. The cost of raw materials used in this method is low, the preparation method is simple, the raw materials are green and safe, and the product does not contain toxic substances. For example, the patent CN110142416A (Yang Boyue, a method for preparing Ag NPs from tangerine peel extract) discloses a method for preparing Ag NPs by using tangerine peel extract as a reducing agent and stabilizer. Some of the effective components in the tangerine peel are adsorbed to the Ag NPs. The surface enhances the stability of Ag NPs. For example, the patent CN105755050A (Wei Xuetuan, a method for biosynthesizing Ag NPs through the extract of octagonal gold plate leaf) discloses a method for biosynthesizing Ag NPs through the extract of octagonal golden disk leaf, but this method requires relatively high reaction conditions. The post-treatment process is complicated. Currently, there are no reports and patent applications on using ginger extract to assist in the preparation of Ag NPs. The preparation method of the present invention is simple and convenient, it has low requirements for experimental operation and reduction at room temperature, and ginger is a 1 common plant, its experimental cost is relatively low, it is environmentally friendly, and is suitable for large-scale preparation of Ag NPs in a short time.
[0004] [0004] Ginger is a traditional Asian medicinal and food homologous plant. Its rhizome is a condiment commonly used in daily cooking. The rhizome, ginger skin and leaves of ginger can be used as medicine. Ginger has a pungent taste, it has the effects of relieving the surface and dispelling cold, warming the lungs and relieving cough and detoxification. It is often used for syndromes of wind-cold, deficiency cold of spleen and stomach. Ginger mainly contains active ingredients such as ginger volatile oil, gingerol and flavonoids. A large number of literature reports that active ingredients such as ginger volatile oil, gingerol and flavonoids all have strong antioxidant properties, it is theoretically possible to make it as a raw material of green reducing agent to prepare Ag NPs. Therefore, the use of rich antioxidant substances in the ginger extract to assist in the preparation of Ag NPs and to explore the antibacterial effect of Ag NPs provides a new way for the development and utilization of ginger resources. Invention Summary The purpose of the present invention is to use ginger extract to assist in the synthesis of Ag NPs and to explore its antibacterial effect. In the present invention, the ginger extract is obtained by the heating reflux extraction method, and then the ginger extract and the silver nitrate solution are mixed and stirred to react to obtain Ag NPs at room temperature, and the antibacterial effect of the Ag NPs is tested, which provides an important reference for the development and utilization of ginger. In order to solve the above technical problems, the present invention provides the following solutions:
[00086] [00086] Further, the mass ratio of ginger powder and distilled water in the above step (2) is 1:15 to 1:50, and the extraction is heated and refluxed for 30~90 min in the range of 60~90 °C;
[0007] [0007] The present invention provides a method for assisting preparation of Ag NPs by 2 ginger extract, which adopts simpler methods and characterization methods to intuitively and accurately reflect the morphology and antibacterial activity of Ag NPs, so as to improve the utilization value of ginger.
[0008] [0008] Description of the drawings: Figure 1 is an X-ray diffraction pattern (XRD) of the product prepared in Embodiment 1.
[0009] [0009] Figure 2 is the ultraviolet absorption spectrum of the Ag NPs synthesized in Embodiment 1 and the comparison experiment silver nitrate and ginger extract.
[0010] [0010] Figure 3 is a scanning electron micrograph (SEM) of the Ag NPs synthesized in Embodiment 1.
[0011] [0011] Figure 4 is a transmission electron microscope image (TEM) of the Ag NPs synthesized in Embodiment 1.
[0012] [0012] Figure 5 shows the antibacterial effect of Ag NPs synthesized in Embodiment 2 on Bacillus subtilis.
[0013] [0013] Figure 6 shows the antibacterial effect of Ag NPs synthesized in Embodiment 2 on Proteus.
[0014] [0014] Detailed Description of the Presently Preferred Embodiments: In the following, the present invention will be specifically described in conjunction with the embodiments: Embodiment 1: assisting preparation of Ag NPs by ginger extract (1) Select the sliced dried ginger and crush, and weigh 4.0 g ginger powder; (2) Select 200 mL of distilled water as the solvent, soak the ginger powder, heat and reflux for 30 min at 80 °C, after cooling, centrifuge and filter to obtain the ginger extract; (3) Prepare 5 mmol/L silver nitrate solution, mix 40 mL of the above ginger extract with 90 mL silver nitrate solution, and stir at room temperature for 36 h; (4) Take the same volume of silver nitrate and ginger extract as a control experiment, and stir at room temperature for 36 h; The sample is characterized by X-ray diffractometer. As shown in Figure 1, it can be seen that it corresponds to the silver standard card (PDF#04-0783), which proves that Ag NPs have been successfully synthesized, The synthesized Ag NPs , the control sample silver nitrate and ginger extract were selected, and the UV absorption curve was measured at 300~700 nm. As shown in Figure 2, it can be seen that the control sample silver nitrate and ginger extract have no absorption peak at 300-700 nm, and the synthesized Ag NPs has a strong absorption peak at 435 nm; A Japanese Hitachi S-4800 cold field emission scanning electron microscope (SEM) is used to characterize the morphology of the sample. As shown in Figure 3, it can be seen that the Ag NPs are in a good dispersed state; 3
[0015] [0015] Embodiment 2: Determination of the antibacterial properties of the Ag NPs synthesized h in Embodiment 1 (1) Inoculate the gram-negative bacterium Proteus and gram-positive bacterium Bacillus subtilis in meat extract peptone medium (LB) respectively, and incubate at 37 °C for 24 h; (2) Then the above two test bacteria are shaken and cultured for 24 h at 37 °C to activate the bacteria to obtain a bacterial suspension; (3) Spread the bacterial suspension evenly on the agar medium plate and dry it, then use sterile tweezers to gently place the autoclaved Oxford cup on the medium; (4) Select 20 pL of Ag NPs synthesized in Embodiment 1 and drop them into the small holes of the Oxford cup, and take the silver nitrate and ginger extracts stirred for the same time in Embodiment 1 as controls, and place them in constant temperature culture at 37 °C. After incubating in the box upside down for 24 h, observe the size of the inhibition zone.
[0016] [0016] Table 1 The diameter of the inhibition zone of the two test bacteria As shown in Figure 5 and Figure 6, it can be seen that the Ag NPs assisted by the ginger extract has inhibitory effects on Proteus and Bacillus subtilis. The diameter of the inhibition zone is measured and compared, and the control can be known from Table 1. The antibacterial effect of the sample silver nitrate and ginger extract is not obvious, and the Ag NPs assisted by the ginger extract has good antibacterial effect. It indicates that the Ag NPs prepared with the aid of ginger extract can exert their potential antibacterial effects in applications such as biomedicine and drug therapy.
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权利要求:
Claims (3)
[1]
1. One kind of preparation method of preparing nanosilver using ginger extract, is characterized by the following process steps: (1) Select the dried ginger powder; (2) With the distilled water as the solvent, add the ginger powder to the distilled water according to a certain mass ratio, and heat and reflux for a certain time within a certain temperature; (3) Suck and filter the supernatant solution, then centrifuge and filter again, and then collect the ginger extract; (4) Take a certain amount of ginger extract and a certain volume of silver nitrate solution of a certain concentration for a certain time at room temperature. Simultaneously, as a control experiment, stir the silver nitrate and ginger extract separately for the same time.
[2]
A kind of preparation method for preparing nanosilver using ginger extract according to claim 1, characterized by: the mass ratio of ginger powder and distilled water in step (2) is from 1:15 to 1:50, and the heating temperature is from 60 up to 90°C, time for extraction is 30 to 120 min.
[3]
A kind of preparation method for preparing nanosilver using ginger extract according to claim 1, characterized by: the amount of ginger extract in step (4) is 20 to 90 ml, and silver nitrate solution is 10 ~ 100 ml with concentration of 1 to 5 mmol/ L, and allow to stir from 12 to 48 hours at room temperature.
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同族专利:

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公开号 | 公开日

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CN111531184A|2020-08-14|

引用文献:

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公开号 | 申请日 | 公开日 | 申请人 | 专利标题

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US6143943A|1998-09-08|2000-11-07|Uop Llc|Process using plate exchanger with high thermal density heat transfer fluid and simultaneous reaction|

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CN112245555A|2020-10-20|2021-01-22|成都中医药大学|Preparation method of dried ginger juice|

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优先权:

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申请号 | 申请日 | 专利标题

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CN202010451635.9A|CN111531184A|2020-05-26|2020-05-26|Method for preparing nano-silver with assistance of ginger extract|

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